Covaris S220 AFA System

Applications

This sonicator operates with Adaptive Focused Acoustics (AFA) technology at high frequencies (500KHz); therefore AFA is inaudible, in contrast to other low frequency sonicators.

It is used for:

• DNA or RNA shearing for e.g. Next Generation Sequencing
• Chromatin shearing
• Tissue homogenization for cell lysis

Details

• Different tubes are available for DNA shearing:

  a) short fragments: micro tubes 100bp-1500bp
(available at GDC)
  b) long fragments: mini tubes: CLEAR for 2kb fragments, BLUE for 3kb fragments, RED for 5kb fragments (can be ordered on request)
• No heat-damage of the sample, as the sonication is done under continuous temperature control and the process runs isothermally.
• No cross-contamination as sonication is done in closed vessels
• Works with accurate, precise and controlled acoustic energy therefore processes and protocols are highly reproducible

Check the external page Covaris website for more details.

Reservation

The Covaris can be reserved via the electronic reservations system. Login with the eth login and password. Use the project number and name when entering reservations and only reserve the exact timeslots needed.