CyFlow Space - Flow Cytometer

Applications

The CyFlow® Space is a bench-top Flow Cytometer designed for cell and microscopic particle analysis and absolute counting (True Absolute Volumetric Counting - TVAC).
It´s a compact multi-colour instrument for analysis of individual cells and microscopic particles in suspension. The advanced flow cytometry technology of the CyFlow Space covers a wide range of different applications.

The GDC instrument however is specifically set up for:

Ploidy level estimation
Genome size estimation
Cell counting

Details

Installed:

Green solid state laser: 532nm
High power UV LED: 365nm

Specification:

Maximum acquisition rate up to 25,000 signals/s
Scatter particle size: 0.1 – 100 µm
Fluorescence sensitivity: < 100 MESF (FITC), < 50 MESF (PE)

Reference genomes available for poidy measurement and genome size estimation:

Drosophila melanogaster breed as reference at GDC. Genome size 1C =0.18pg, Megabases haploid: 176 MB
Chicken Red Blood Cells (CRBC). Tissue: Erythrocytes from a female, in freezing medium for live cells (DMEM plus DMSO). Genome size 1C =1.075, Megabases haploid: 1051.35 Mb
Trout. Tissue: red blood cells. However, should not be used as a standard for genome size determination according to Labforce as cells are fixed and stained. 2C: 4.9 to 6.3 pg for various of trout, Megabases unknown.

Staining kits available:

Cystain UV ploidy (DAPI colour used with UV)
Propodium Iodid (PI used with green laser)

Check the external page Sysmex website for more details.

Reservation

The CyFlow Space can be reserved via the electronic reservations system. Login with the eth login and password. Use the project number and name when entering reservations and only reserve the exact timeslots needed.